other:
cDNA Synthesis:Article Title: Tuft cell acetylcholine is released into the gut lumen to promote anti-helminth immunity.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER PBS 1X (Ca2+ et Mg2+ free) Eurobio Cat# CS1PB01-01 LiberaseTM Roche Cat# 5401119001 EDTA Sigma-Aldrich Cat# 03690-100 DTT Sigma-Aldrich Cat# 43816-50 Vetflurane 1000 mg/g Virbac GTIN 03597132002653 Buprecare 0.3 mg/ml Axience GTIN 03760087151244 Tamoxifene Sigma-Aldrich Cat# T5648 Laocaine 16mg MSD GTIN: 05017363520132 Trizol reagent Thermo Fisher Cat# 15596026 Buprenorphine Sigma-Aldrich Cat#B9275 Hematoxylin Solution, Gill No. 3 Sigma-Aldrich Cat#GHS316 SIGMAFAST 3,30-Diaminobenzidine tablets Sigma-Aldrich Cat# D4293 N-Histofine Nichirei Biosciences 414151F Critical commercial assays ATPLite kit Perkin Elmer Cat# 6016943 RNeasy Micro Kit Qiagen ID: 74004 RNeasy Mini Kit Qiagen ID: 74104 Transcriptor First Strand cDNA Synthesis Kit Roche lifescience Cat# 04896866001 LightCycler 480 SYBR Green I Master Roche lifescience Cat# 04887352001 RNAscope Multiplex Fluorescent V2 Assay ACD a biotechne brand Cat# 323270 Fix/perm kit FOXP3/transcription factor staining buffer set eBioscience/ThermoFisher Cat# 00-5523-00 Arcturus RiboAmp Plus kit Thermo Fisher Cat# KIT0501 Experimental models: Organisms/strains Mouse strain Chattm1Mlt Lecomte et al.22 Provided by S. Berrard Mouse strain Tg(Vil1-cre/ERT2)23Syr El Marjour et al.23 Provided by S. Robine Mouse strain ChatLoxP/LoxP; VillinCreERT2/+ This study N/A Heligmosomoides polygyrus Provided by R. Maizels Provided by R. Maizels Oligonucleotides Chat Forward TTTGCAGCCAGCCTCATCTC Reverse TATGGCCAGGAAGCCGGTAT Eurofins VAChT Forward GGCCTCGCTACCCCACAGAA Reverse CCCAGGCCAATAAGCAGCGG Eurofins Pou2f3 Forward GAGGGAATGATGAGCCCACT Reverse GTGAAGCCTAGCTTAATGCGTC Eurofins Alox5 Forward TCTTCCTGGCACGACTTTGCTG Reverse GCAGCCATTCAGGAACTGGTAG Eurofins Alox5ap Forward GTTCTTTGCCCACAAGGTGGAG Reverse TGCAGTCCAGAGTACCACAAGG Eurofins Ptgs1 Forward GAATGCCACCTTCATCCGAGAAG Reverse GCTCACATTGGAGAAGGACTCC Eurofins Ptgs2 Forward TCAATGAGTACCGCAAACGC Reverse AGGGTACAGTTCCATGACATCG Eurofins Hpgds Forward GAATAGAACAAGCTGACTGGC Reverse AGCCAAATCTGTGTTTTTGG Eurofins Ltc4s Forward CCTACAGGTGATCTCTGCACGA Reverse TGGCGAGGAACAGCGGAAAGTA Eurofins Sucnr1 Forward CCATCTCTGACTTTGCTTTCCTG Reverse GTGTAGAGGTTGGTGTGAAGCAC Eurofins Dclk1 Forward CAGCCTGGACGAGCTGGTGG Reverse TGACCAGTTGGGGTTCACAT Eurofins (Continued on next page) e3 Immunity 57, 1260–1273.e1–e7, June 11, 2024 .. REAGENT or RESOURCE SOURCE IDENTIFIER Il25 Forward CCTGTCAGGCAGGGGTAGTA Reverse CCAAGAATGCAACAGCCTG Eurofins Villin Forward: CAAGCCTGGCTCGACGGCC Reverse: CGCGAACATCTTCAGGTTCT Eurofins Chat (exon8) Forward: CCCGTTTTCCTTCCAGCCAA Reverse: CGCGAACATCTTCAGGTTCT Eurofins Gapdh Forward GGAGCGAGACCCCACTAACA Reverse ACATACTCAGCACCGGCCTC Eurofins Hprt Forward GCAGTACAGCCCCAAAATGG Reverse GGTCCTTTTCACCAGCAAGCT Eurofins Software and algorithms NdpView.V2 Hamamatsu Photonics https://www.hamamatsu.com Zen blue edition.
SYBR Green Assay:Article Title: Tuft cell acetylcholine is released into the gut lumen to promote anti-helminth immunity.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER PBS 1X (Ca2+ et Mg2+ free) Eurobio Cat# CS1PB01-01 LiberaseTM Roche Cat# 5401119001 EDTA Sigma-Aldrich Cat# 03690-100 DTT Sigma-Aldrich Cat# 43816-50 Vetflurane 1000 mg/g Virbac GTIN 03597132002653 Buprecare 0.3 mg/ml Axience GTIN 03760087151244 Tamoxifene Sigma-Aldrich Cat# T5648 Laocaine 16mg MSD GTIN: 05017363520132 Trizol reagent Thermo Fisher Cat# 15596026 Buprenorphine Sigma-Aldrich Cat#B9275 Hematoxylin Solution, Gill No. 3 Sigma-Aldrich Cat#GHS316 SIGMAFAST 3,30-Diaminobenzidine tablets Sigma-Aldrich Cat# D4293 N-Histofine Nichirei Biosciences 414151F Critical commercial assays ATPLite kit Perkin Elmer Cat# 6016943 RNeasy Micro Kit Qiagen ID: 74004 RNeasy Mini Kit Qiagen ID: 74104 Transcriptor First Strand cDNA Synthesis Kit Roche lifescience Cat# 04896866001 LightCycler 480 SYBR Green I Master Roche lifescience Cat# 04887352001 RNAscope Multiplex Fluorescent V2 Assay ACD a biotechne brand Cat# 323270 Fix/perm kit FOXP3/transcription factor staining buffer set eBioscience/ThermoFisher Cat# 00-5523-00 Arcturus RiboAmp Plus kit Thermo Fisher Cat# KIT0501 Experimental models: Organisms/strains Mouse strain Chattm1Mlt Lecomte et al.22 Provided by S. Berrard Mouse strain Tg(Vil1-cre/ERT2)23Syr El Marjour et al.23 Provided by S. Robine Mouse strain ChatLoxP/LoxP; VillinCreERT2/+ This study N/A Heligmosomoides polygyrus Provided by R. Maizels Provided by R. Maizels Oligonucleotides Chat Forward TTTGCAGCCAGCCTCATCTC Reverse TATGGCCAGGAAGCCGGTAT Eurofins VAChT Forward GGCCTCGCTACCCCACAGAA Reverse CCCAGGCCAATAAGCAGCGG Eurofins Pou2f3 Forward GAGGGAATGATGAGCCCACT Reverse GTGAAGCCTAGCTTAATGCGTC Eurofins Alox5 Forward TCTTCCTGGCACGACTTTGCTG Reverse GCAGCCATTCAGGAACTGGTAG Eurofins Alox5ap Forward GTTCTTTGCCCACAAGGTGGAG Reverse TGCAGTCCAGAGTACCACAAGG Eurofins Ptgs1 Forward GAATGCCACCTTCATCCGAGAAG Reverse GCTCACATTGGAGAAGGACTCC Eurofins Ptgs2 Forward TCAATGAGTACCGCAAACGC Reverse AGGGTACAGTTCCATGACATCG Eurofins Hpgds Forward GAATAGAACAAGCTGACTGGC Reverse AGCCAAATCTGTGTTTTTGG Eurofins Ltc4s Forward CCTACAGGTGATCTCTGCACGA Reverse TGGCGAGGAACAGCGGAAAGTA Eurofins Sucnr1 Forward CCATCTCTGACTTTGCTTTCCTG Reverse GTGTAGAGGTTGGTGTGAAGCAC Eurofins Dclk1 Forward CAGCCTGGACGAGCTGGTGG Reverse TGACCAGTTGGGGTTCACAT Eurofins (Continued on next page) e3 Immunity 57, 1260–1273.e1–e7, June 11, 2024 .. REAGENT or RESOURCE SOURCE IDENTIFIER Il25 Forward CCTGTCAGGCAGGGGTAGTA Reverse CCAAGAATGCAACAGCCTG Eurofins Villin Forward: CAAGCCTGGCTCGACGGCC Reverse: CGCGAACATCTTCAGGTTCT Eurofins Chat (exon8) Forward: CCCGTTTTCCTTCCAGCCAA Reverse: CGCGAACATCTTCAGGTTCT Eurofins Gapdh Forward GGAGCGAGACCCCACTAACA Reverse ACATACTCAGCACCGGCCTC Eurofins Hprt Forward GCAGTACAGCCCCAAAATGG Reverse GGTCCTTTTCACCAGCAAGCT Eurofins Software and algorithms NdpView.V2 Hamamatsu Photonics https://www.hamamatsu.com Zen blue edition.
RNAscope:Article Title: Tuft cell acetylcholine is released into the gut lumen to promote anti-helminth immunity.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER PBS 1X (Ca2+ et Mg2+ free) Eurobio Cat# CS1PB01-01 LiberaseTM Roche Cat# 5401119001 EDTA Sigma-Aldrich Cat# 03690-100 DTT Sigma-Aldrich Cat# 43816-50 Vetflurane 1000 mg/g Virbac GTIN 03597132002653 Buprecare 0.3 mg/ml Axience GTIN 03760087151244 Tamoxifene Sigma-Aldrich Cat# T5648 Laocaine 16mg MSD GTIN: 05017363520132 Trizol reagent Thermo Fisher Cat# 15596026 Buprenorphine Sigma-Aldrich Cat#B9275 Hematoxylin Solution, Gill No. 3 Sigma-Aldrich Cat#GHS316 SIGMAFAST 3,30-Diaminobenzidine tablets Sigma-Aldrich Cat# D4293 N-Histofine Nichirei Biosciences 414151F Critical commercial assays ATPLite kit Perkin Elmer Cat# 6016943 RNeasy Micro Kit Qiagen ID: 74004 RNeasy Mini Kit Qiagen ID: 74104 Transcriptor First Strand cDNA Synthesis Kit Roche lifescience Cat# 04896866001 LightCycler 480 SYBR Green I Master Roche lifescience Cat# 04887352001 RNAscope Multiplex Fluorescent V2 Assay ACD a biotechne brand Cat# 323270 Fix/perm kit FOXP3/transcription factor staining buffer set eBioscience/ThermoFisher Cat# 00-5523-00 Arcturus RiboAmp Plus kit Thermo Fisher Cat# KIT0501 Experimental models: Organisms/strains Mouse strain Chattm1Mlt Lecomte et al.22 Provided by S. Berrard Mouse strain Tg(Vil1-cre/ERT2)23Syr El Marjour et al.23 Provided by S. Robine Mouse strain ChatLoxP/LoxP; VillinCreERT2/+ This study N/A Heligmosomoides polygyrus Provided by R. Maizels Provided by R. Maizels Oligonucleotides Chat Forward TTTGCAGCCAGCCTCATCTC Reverse TATGGCCAGGAAGCCGGTAT Eurofins VAChT Forward GGCCTCGCTACCCCACAGAA Reverse CCCAGGCCAATAAGCAGCGG Eurofins Pou2f3 Forward GAGGGAATGATGAGCCCACT Reverse GTGAAGCCTAGCTTAATGCGTC Eurofins Alox5 Forward TCTTCCTGGCACGACTTTGCTG Reverse GCAGCCATTCAGGAACTGGTAG Eurofins Alox5ap Forward GTTCTTTGCCCACAAGGTGGAG Reverse TGCAGTCCAGAGTACCACAAGG Eurofins Ptgs1 Forward GAATGCCACCTTCATCCGAGAAG Reverse GCTCACATTGGAGAAGGACTCC Eurofins Ptgs2 Forward TCAATGAGTACCGCAAACGC Reverse AGGGTACAGTTCCATGACATCG Eurofins Hpgds Forward GAATAGAACAAGCTGACTGGC Reverse AGCCAAATCTGTGTTTTTGG Eurofins Ltc4s Forward CCTACAGGTGATCTCTGCACGA Reverse TGGCGAGGAACAGCGGAAAGTA Eurofins Sucnr1 Forward CCATCTCTGACTTTGCTTTCCTG Reverse GTGTAGAGGTTGGTGTGAAGCAC Eurofins Dclk1 Forward CAGCCTGGACGAGCTGGTGG Reverse TGACCAGTTGGGGTTCACAT Eurofins (Continued on next page) e3 Immunity 57, 1260–1273.e1–e7, June 11, 2024 .. REAGENT or RESOURCE SOURCE IDENTIFIER Il25 Forward CCTGTCAGGCAGGGGTAGTA Reverse CCAAGAATGCAACAGCCTG Eurofins Villin Forward: CAAGCCTGGCTCGACGGCC Reverse: CGCGAACATCTTCAGGTTCT Eurofins Chat (exon8) Forward: CCCGTTTTCCTTCCAGCCAA Reverse: CGCGAACATCTTCAGGTTCT Eurofins Gapdh Forward GGAGCGAGACCCCACTAACA Reverse ACATACTCAGCACCGGCCTC Eurofins Hprt Forward GCAGTACAGCCCCAAAATGG Reverse GGTCCTTTTCACCAGCAAGCT Eurofins Software and algorithms NdpView.V2 Hamamatsu Photonics https://www.hamamatsu.com Zen blue edition.
Multiplex Assay:Article Title: Tuft cell acetylcholine is released into the gut lumen to promote anti-helminth immunity.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER PBS 1X (Ca2+ et Mg2+ free) Eurobio Cat# CS1PB01-01 LiberaseTM Roche Cat# 5401119001 EDTA Sigma-Aldrich Cat# 03690-100 DTT Sigma-Aldrich Cat# 43816-50 Vetflurane 1000 mg/g Virbac GTIN 03597132002653 Buprecare 0.3 mg/ml Axience GTIN 03760087151244 Tamoxifene Sigma-Aldrich Cat# T5648 Laocaine 16mg MSD GTIN: 05017363520132 Trizol reagent Thermo Fisher Cat# 15596026 Buprenorphine Sigma-Aldrich Cat#B9275 Hematoxylin Solution, Gill No. 3 Sigma-Aldrich Cat#GHS316 SIGMAFAST 3,30-Diaminobenzidine tablets Sigma-Aldrich Cat# D4293 N-Histofine Nichirei Biosciences 414151F Critical commercial assays ATPLite kit Perkin Elmer Cat# 6016943 RNeasy Micro Kit Qiagen ID: 74004 RNeasy Mini Kit Qiagen ID: 74104 Transcriptor First Strand cDNA Synthesis Kit Roche lifescience Cat# 04896866001 LightCycler 480 SYBR Green I Master Roche lifescience Cat# 04887352001 RNAscope Multiplex Fluorescent V2 Assay ACD a biotechne brand Cat# 323270 Fix/perm kit FOXP3/transcription factor staining buffer set eBioscience/ThermoFisher Cat# 00-5523-00 Arcturus RiboAmp Plus kit Thermo Fisher Cat# KIT0501 Experimental models: Organisms/strains Mouse strain Chattm1Mlt Lecomte et al.22 Provided by S. Berrard Mouse strain Tg(Vil1-cre/ERT2)23Syr El Marjour et al.23 Provided by S. Robine Mouse strain ChatLoxP/LoxP; VillinCreERT2/+ This study N/A Heligmosomoides polygyrus Provided by R. Maizels Provided by R. Maizels Oligonucleotides Chat Forward TTTGCAGCCAGCCTCATCTC Reverse TATGGCCAGGAAGCCGGTAT Eurofins VAChT Forward GGCCTCGCTACCCCACAGAA Reverse CCCAGGCCAATAAGCAGCGG Eurofins Pou2f3 Forward GAGGGAATGATGAGCCCACT Reverse GTGAAGCCTAGCTTAATGCGTC Eurofins Alox5 Forward TCTTCCTGGCACGACTTTGCTG Reverse GCAGCCATTCAGGAACTGGTAG Eurofins Alox5ap Forward GTTCTTTGCCCACAAGGTGGAG Reverse TGCAGTCCAGAGTACCACAAGG Eurofins Ptgs1 Forward GAATGCCACCTTCATCCGAGAAG Reverse GCTCACATTGGAGAAGGACTCC Eurofins Ptgs2 Forward TCAATGAGTACCGCAAACGC Reverse AGGGTACAGTTCCATGACATCG Eurofins Hpgds Forward GAATAGAACAAGCTGACTGGC Reverse AGCCAAATCTGTGTTTTTGG Eurofins Ltc4s Forward CCTACAGGTGATCTCTGCACGA Reverse TGGCGAGGAACAGCGGAAAGTA Eurofins Sucnr1 Forward CCATCTCTGACTTTGCTTTCCTG Reverse GTGTAGAGGTTGGTGTGAAGCAC Eurofins Dclk1 Forward CAGCCTGGACGAGCTGGTGG Reverse TGACCAGTTGGGGTTCACAT Eurofins (Continued on next page) e3 Immunity 57, 1260–1273.e1–e7, June 11, 2024 .. REAGENT or RESOURCE SOURCE IDENTIFIER Il25 Forward CCTGTCAGGCAGGGGTAGTA Reverse CCAAGAATGCAACAGCCTG Eurofins Villin Forward: CAAGCCTGGCTCGACGGCC Reverse: CGCGAACATCTTCAGGTTCT Eurofins Chat (exon8) Forward: CCCGTTTTCCTTCCAGCCAA Reverse: CGCGAACATCTTCAGGTTCT Eurofins Gapdh Forward GGAGCGAGACCCCACTAACA Reverse ACATACTCAGCACCGGCCTC Eurofins Hprt Forward GCAGTACAGCCCCAAAATGG Reverse GGTCCTTTTCACCAGCAAGCT Eurofins Software and algorithms NdpView.V2 Hamamatsu Photonics https://www.hamamatsu.com Zen blue edition.
Staining:Article Title: Tuft cell acetylcholine is released into the gut lumen to promote anti-helminth immunity.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER PBS 1X (Ca2+ et Mg2+ free) Eurobio Cat# CS1PB01-01 LiberaseTM Roche Cat# 5401119001 EDTA Sigma-Aldrich Cat# 03690-100 DTT Sigma-Aldrich Cat# 43816-50 Vetflurane 1000 mg/g Virbac GTIN 03597132002653 Buprecare 0.3 mg/ml Axience GTIN 03760087151244 Tamoxifene Sigma-Aldrich Cat# T5648 Laocaine 16mg MSD GTIN: 05017363520132 Trizol reagent Thermo Fisher Cat# 15596026 Buprenorphine Sigma-Aldrich Cat#B9275 Hematoxylin Solution, Gill No. 3 Sigma-Aldrich Cat#GHS316 SIGMAFAST 3,30-Diaminobenzidine tablets Sigma-Aldrich Cat# D4293 N-Histofine Nichirei Biosciences 414151F Critical commercial assays ATPLite kit Perkin Elmer Cat# 6016943 RNeasy Micro Kit Qiagen ID: 74004 RNeasy Mini Kit Qiagen ID: 74104 Transcriptor First Strand cDNA Synthesis Kit Roche lifescience Cat# 04896866001 LightCycler 480 SYBR Green I Master Roche lifescience Cat# 04887352001 RNAscope Multiplex Fluorescent V2 Assay ACD a biotechne brand Cat# 323270 Fix/perm kit FOXP3/transcription factor staining buffer set eBioscience/ThermoFisher Cat# 00-5523-00 Arcturus RiboAmp Plus kit Thermo Fisher Cat# KIT0501 Experimental models: Organisms/strains Mouse strain Chattm1Mlt Lecomte et al.22 Provided by S. Berrard Mouse strain Tg(Vil1-cre/ERT2)23Syr El Marjour et al.23 Provided by S. Robine Mouse strain ChatLoxP/LoxP; VillinCreERT2/+ This study N/A Heligmosomoides polygyrus Provided by R. Maizels Provided by R. Maizels Oligonucleotides Chat Forward TTTGCAGCCAGCCTCATCTC Reverse TATGGCCAGGAAGCCGGTAT Eurofins VAChT Forward GGCCTCGCTACCCCACAGAA Reverse CCCAGGCCAATAAGCAGCGG Eurofins Pou2f3 Forward GAGGGAATGATGAGCCCACT Reverse GTGAAGCCTAGCTTAATGCGTC Eurofins Alox5 Forward TCTTCCTGGCACGACTTTGCTG Reverse GCAGCCATTCAGGAACTGGTAG Eurofins Alox5ap Forward GTTCTTTGCCCACAAGGTGGAG Reverse TGCAGTCCAGAGTACCACAAGG Eurofins Ptgs1 Forward GAATGCCACCTTCATCCGAGAAG Reverse GCTCACATTGGAGAAGGACTCC Eurofins Ptgs2 Forward TCAATGAGTACCGCAAACGC Reverse AGGGTACAGTTCCATGACATCG Eurofins Hpgds Forward GAATAGAACAAGCTGACTGGC Reverse AGCCAAATCTGTGTTTTTGG Eurofins Ltc4s Forward CCTACAGGTGATCTCTGCACGA Reverse TGGCGAGGAACAGCGGAAAGTA Eurofins Sucnr1 Forward CCATCTCTGACTTTGCTTTCCTG Reverse GTGTAGAGGTTGGTGTGAAGCAC Eurofins Dclk1 Forward CAGCCTGGACGAGCTGGTGG Reverse TGACCAGTTGGGGTTCACAT Eurofins (Continued on next page) e3 Immunity 57, 1260–1273.e1–e7, June 11, 2024 .. REAGENT or RESOURCE SOURCE IDENTIFIER Il25 Forward CCTGTCAGGCAGGGGTAGTA Reverse CCAAGAATGCAACAGCCTG Eurofins Villin Forward: CAAGCCTGGCTCGACGGCC Reverse: CGCGAACATCTTCAGGTTCT Eurofins Chat (exon8) Forward: CCCGTTTTCCTTCCAGCCAA Reverse: CGCGAACATCTTCAGGTTCT Eurofins Gapdh Forward GGAGCGAGACCCCACTAACA Reverse ACATACTCAGCACCGGCCTC Eurofins Hprt Forward GCAGTACAGCCCCAAAATGG Reverse GGTCCTTTTCACCAGCAAGCT Eurofins Software and algorithms NdpView.V2 Hamamatsu Photonics https://www.hamamatsu.com Zen blue edition.
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Roche manufactures this product 